On the defensive action of glutamate against the cytotoxicity and fibrogenicity of quartz dust.

نویسندگان

  • K I Morosova
  • G V Aronova
  • B A Katsnelson
  • B T Velichkovski
  • A M Genkin
  • L N Elnichnykh
  • L I Privalova
چکیده

The cytotoxic action of quartz (DQ12) particles on cultures of rat peritoneal macrophages, as estimated by the inhibition of the TTC-reductase activity, is considerably reduced by preincubation with glutamic acid and by adding sodium glutamate (15 mg/ml) to the drinking water of the rats donating the macrophages. This increase in macrophage resistance under the influence of glutamate is the most probable cause of the delay in the development of silicotic fibrosis shown in several experiments on rats intratracheally injected with quartz and then treated by prolonged administration of glutamate. This effect is probably connected with the influence of glutamate on the stability of the macrophage membranes, which can in its turn be explained by different mechanisms, including the influence on the synthesis and phosphorylation of adenosine nucleotides. Such an influence was shown in rats receiving glutamate by the change of the ATP/ADP ratio in macrophages, but not in erythrocytes. The resistance of rat erythrocytes to the haemolytic action of quartz is also not influenced by the action of glutamate neither in vitro nor in vivo. Such differences in the influences of glutamate on two types of cells, equally susceptible to quartz cytotoxicity but considerably differing in the character of energy metabolism, is an indirect proof of the role of the latter in the realisation of the anticytotoxic, and thereby antifibrogenic, effect of glutamate.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The fibrogenicity of some respirable dusts measured in mice.

A method of bio-assay of the fibrogenicity of respirable dust is described. Quartz, coal, and quartz-coal dust mixtures were administered to mice via the tail vein, and liver collagen was estimated chemically. Groups of mice that had received doses of 10, 15, and 20 mg. of these dusts were killed at intervals of 10, 20, and 30 weeks after injection. There was, in general, a linear relation betw...

متن کامل

Prevalence and pathogenesis of pneumoconiosis in coal workers.

Dust dose and composition do not appear to account wholly for changes in the prevalence of coal workers' pneumoconiosis in Europe. In certain coal pits high progression evidently occurred with relatively low dust exposure or vice versa, whereas progression in relation to dust levels might be variable. Exceptionally high quartz concentrations occur in coal mine dust when pneumoconiosis may progr...

متن کامل

Berberine protects against glutamate-induced oxidative stress and apoptosis in PC12 and N2a cells

Objective(s): Neurodegenerative diseases have been associated with glutamatergic dysfunction. Berberine, an isoquinoline alkaloid broadly present in different medicinal herbs, has been reported to have neuroprotective effect. In the present study, the effects of berberine against glutamate-induced oxidative damage and apoptosis were investigated. Materials and Methods: The cultured PC12 and N2a...

متن کامل

ارزیابی مواجهه شغلی کارگران با سیلیس آزاد و گردوغبار کل در کارگاه حفر تونل زیر زمینی متروی تهران  خط 1     

Introduction: Exposure to crystalline silica in the construction workers such as miners and diggers are widespread. Exposure to respirable silica (quartz) can results in silicosis. The goal of this study was to determine the contribution of each workers and component task to total dust and respirable quartz exposure in the tunnel of metro. .Method and Materials: The assessment of crystallin...

متن کامل

Exploring the Protective Effects of Abrus precatorius in HepG2 and N-Nitrosodiethylamine-Induced Hepatocellular Carcinoma in Swiss Albino Rats

      This study was designed to explore the protective effects of Abrus precatorius L. (Leguminosae) (AP) in HepG2 cells and N-nitrosodiethylamine (NDEA) induced hepatocellular carcinoma in Swiss albino rats. The effects of aqueous/ethanolic (50%) extract of AP on hepatic markers, haematological and histopathological parameters, and antioxidant enzymes were evaluated in NDEA ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • British journal of industrial medicine

دوره 39 3  شماره 

صفحات  -

تاریخ انتشار 1982